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Asian Journal of Pharmaceutical Research
Year : 2019, Volume : 9, Issue : 3
First page : ( 169) Last page : ( 171)
Print ISSN : 2231-5683. Online ISSN : 2231-5691.
Article DOI : 10.5958/2231-5691.2019.00026.1

Membrane Stabilization assay for Anti-inflammatory activity yields misleading results for samples containing traces of Methanol

Navale Govind1, Patil Dipak D.3, Patil Aashutosh A.2, Patil Ketan B.2, Patil Narendra B.2,*

1Department of Bioanalytical, Sitec Labs Pvt Ltd., Mahape (Navi Mumbai)

2Deprtment of Pharmacology, Ahinsa Institute of Pharmacy, Dhule-Road, Dondaicha

3Department of Quality Assurance, H. R. Patel Institute of Pharmaceutical Education and Research, Shirpur

*Corresponding Author E-mail: pnarendra101@gmail.com

Online published on 31 December, 2019.


There is an increased in the reports on erythrocyte haemolysis assay being used as a simple and rapid tool for screening of drugs and phytochemicals for anti-inflammatory activity. However, in case of hydroalchoholic extracts, there is a possibility of the trace amounts of methanol retained even after drying. These traces are not acconted in the assay units. The present study was designated to substantiate the effects of low concentrations of methanol on the heat induced haemolysis. It was observed that the presence of methanol at concentrations as low as 0.1 l/ml exert significant membrane stabilizing activity. Thus, this assay may give misleading results if the test drug solution contains even slighest amount of methanol. Therefore, Present study suggested that the heatinduced erythrocyte haemolysis assay is not suitable for testing membrane stabilizing and anti-inflammmatory activity of the test samples containing methanol.



Methanol, erythrocyte haemolysis, Membrane stabilization, RBC haemolysis, Ibuprofen.


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