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Asian Journal of Pharmaceutical Research
Year : 2018, Volume : 8, Issue : 4
First page : ( 225) Last page : ( 230)
Print ISSN : 2231-5683. Online ISSN : 2231-5691.
Article DOI : 10.5958/2231-5691.2018.00038.2

Autolysis activity of Vancomycin Resistance Staphylococcus epidermidis

Abdrabaa Mariam K.1,*, Flayyih May T.2

1Department of Medical Lab. Technology, Al-Esraa University College

2Department of Biology, College of Science, University of Baghdad. Baghdad, Iraq

*Corresponding Author E-mail: mariam_khamees@yahoo.com

Online published on 25 December, 2018.

Abstract

The effect of vancomycin resistant on cell autolysis activity of Staphylococcus epidermidis was detected by whole cell autolytic assay. Three isolates of S. epidermidis, vancomycin sensitive (VSSE), vancomycin resistance (VRSE) and vancomycin intermediate (VISE) were tested. The results revealed that there was significant difference among three isolates, the VSSE isolate (S.epidermidis 22) have the highest autolytic activity in the presence of antibiotic, followed by the VRSE isolate (S. epidermidis 1) and the VISE isolate (S. epidermidis14) which was the lowest autolytic activity with the presence of antibiotic. The result of transmission electron microscope (TEM) showed that the VRSE isolates (S. epidermidis 1) have thicker cell wall followed by VISE (S. epidermidis 14) isolates. However, the VSSE (S. epidermidis 22) didn't showed any cell wall thickening

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Keywords

Staphylococcus epidermidis, vancomycin resistant, autolysin, VRSE, VISE, VSSE.

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