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Asian Journal of Pharmaceutical Analysis
Year : 2019, Volume : 9, Issue : 3
First page : ( 138) Last page : ( 150)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2019.00026.7

Development and Validation of UV Spectroscopic by Q-absorption Ratio, RP-HPLC Method for Simultaneous Estimation of Atazanvir and Ritonavir in bulk and Pharmaceutical Dosage Form

Sri K. Vijaya*, Deepthi S., Madhuri M., Aishwarya P. V.

Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, (Affiliated to Osmania University) Maisammaguda, Secunderabad-500 100

*Corresponding Author E-mail: vijayasree_2002@yahoo.co.in

Online published on 31 December, 2019.


The main objectives of presented work were developed and validate the UV-spectrophotometric by Q-absorption ratio and RP-HPLC method for the simultaneous estimation of Atazanavir and Ritonavir as per ICH guidelines in bulk and pharmaceutical dosage form. In UV-spectrophotometric, RP-HPLC method was developed by using Acetonitrile used as a solvent and Acetonitrile/acetate buffer in the ratio of 60: 40(%v/v) used as a mobile phase. The RP-HPLC method was developed in Eclipse C18 column (100 mm × 4.6 mm, 3.5 μm particle size). In UVspectrophotometric method, the λmax of Atazanavir and Ritonavir was 250 and 239.4nm (iso absorption point) and for HPLC-UV detection at 250 nm were found to be respectively. It was proved that linearity in the concentration range of 5–35 μg/ml for Atazanavir, 10–60 μg/ml for Ritonavir and for HPLC the linearity range between 3–150μg/ml for Atazanavir and Ritonavir 1–50 μg/ml with a correlation coefficient value of 0.999. The % RSD for intraday precision and interday precision was less than 2% for UV-spectrophotometric and RP-HPLC method. A new, simple, accurate UV-spectrophotometric and RP-HPLC method were developed for the simultaneous estimation of Atazanavir and Ritonavir in the bulk and in the pharmaceutical dosage form.



Atazanavir, Ritonavir, Q-absorption ratio, RP-HPLC and ICH.


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