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Asian Journal of Pharmaceutical Analysis
Year : 2019, Volume : 9, Issue : 2
First page : ( 49) Last page : ( 54)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2019.00011.5

Development and Validation of Zero and First-Order Derivative Area under Curve Spectrophotometric Methods for the Determination of Deferiprone in Bulk Material and Capsules

Malik Aamir1, Firke Sandip2,*, Patil Ravindra2, Shirkhedkar Atul2, Kalaskar Mohan2

1Research Scholar Department, R. C. Patel Institute of Pharmaceutical Education and Research, Shipur, Dhule (425405)

2Professor, Pharmaceutical Chemistry Department, R. C. Patel Institute of Pharmaceutical Education and Research, Shipur, Dhule (425405)

*Corresponding Author E-mail: sandipfirke@rediffmail.com

Online published on 3 July, 2019.


A simple, accurate and precise spectrophotometric method including area under curve has been developed for the estimation of Deferiprone (DEF) in bulk material and capsule dosage form. Deferiprone is an iron chelating agent, used in the treatment of thalassemia syndromes when iron overload due to blood transfusions occurs. Four simple UV Spectrophotometric methods were established for determination of Deferiprone, using double beam UV spectrophotometer (UV-2450, Shimadzu, Japan). Deferiprone has absorbance maxima at 278 nm with distilled water used as a solvent. Deferiprone obeys Beer-Lambert's law in the concentration range of 2–10 μg/mL. The % recovery by proposed methods was found to be 98–101%. The Precision studies show less than 2 in terms of %RSD indicates precise nature of proposed method. All methods were validated as per the ICH guidelines, Q2 (R1). The statistical data and results prove that developed methods are simple, accurate and precise and can be used for the routine analysis of DEF in bulk and pharmaceutical formulation.



Deferiprone (DEF), AUC, UV-spectrophotometer, Derivative spectroscopy.


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