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Asian Journal of Pharmaceutical Analysis
Year : 2019, Volume : 9, Issue : 1
First page : ( 11) Last page : ( 14)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2019.00004.8

Development and Validation of UV-Spectrophotometric method for the Determination of Enrofloxacin in Synthetic form and Veterinary Injectible Dosage forms

Elmahadii Tasneem O.2, Shantier Shaza W. A1, Adam Mohamed E.1,*

1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, Sudan

2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Omdurman Islamic University, Omdurman, Sudan

*Corresponding Author E-mail: adamo86073@gmail.com

Online published on 15 March, 2019.


Simple, sensitive and accurate spectrophotometric method was developed for the estimation of Enrofloxacin (EFC) in bulk and injection dosage form. The method was based on the measurement of EFC solution in 0.1M potassium hydroxide at 271nm. Validation was conducted in accordance to ICH guidelines. The developed method was found to be linear in the concentration range 2–10 μg/ml (r= 0.999), sensitive (LOD was found 0.127 μg/ml) and precise (RSD% values are less than 2%). Freedom from interference by the formulation excipients was proved by the good recovery percentages’ results (100.06% ± 1.12, n=3). The proposed method was successfully used to estimate EFC in laboratory mixture and in the injection dosage forms.



Enrofloxacin, spectrophotometric method, Validation, Injections.


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