(3.236.225.157)
Users online: 3183    [ij] [ij] [ij] 
Email id
 

Asian Journal of Pharmaceutical Analysis
Year : 2018, Volume : 8, Issue : 3
First page : ( 153) Last page : ( 156)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2018.00028.5

Method Development and Validation for Simultaneous Estimation of Omeprazole and Ofloxacin by using RP-HPLC

Akula Ganesh1,*, Rajashekar P.1, Nirmala Rangu2, Kanakaiah K.3, Dr. Jaswanth A.3

1Department of Pharmaceutical Chemistry, Surabhi Dayakar Rao College of Pharmacy, Rimmanaguda, Gajwel, Siddipet, Telangana-502312

2Department of Pharmaceutics, Surabhi Dayakar Rao College of Pharmacy, Rimmanaguda, Gajwel, Siddipet, Telangana

3Department of Pharmacology, Surabhi Dayakar Rao College of Pharmacy, Rimmanaguda, Gajwel, Siddipet, Telangana

*Corresponding Author E-mail: akulaganesh@gmail.com

Online published on 24 October, 2018.

Abstract

A simple, Accurate, precise Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) method was developed for the simultaneous estimation of the omeprazole and Ofloxacin in Tablet dosage form. Chromatogram was run through ODS (150X4.6mm, 5μ). Mobile phase containing Buffer and Acetonitrile in the ratio of 45: 55 was pumped through column at a flow rate of 0.8 ml/min. Buffer temperature was maintained at 30°C. Optimized wavelength for Omeprazole and Ofloxacin was 220nm. Retention time of Omeprazole and Ofloxacin were found to be 2.16 min and 3.39 min. %RSD of the Omeprazole and Ofloxacin were and found to be 0.62 and 0.74 respectively. %Recover was Obtained as 100.07% and 100.72% for Omeprazole and Ofloxacin. LOD, LOQ values were obtained from regression equations of Omeprazole and Ofloxacin were 0.27ppm, 0.37ppm and 0.83ppm, 1.13ppm respectively. Regression equation of Omeprazole is y=11878x+281.6, and of Ofloxacin is y=14453x+3910.2.

Top

Keywords

Omeprazole, Ofloxacin, RP-HPLC, LOD, LOQ.

Top

  
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
576,316,101 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.