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Asian Journal of Pharmaceutical Analysis
Year : 2017, Volume : 7, Issue : 3
First page : ( 189) Last page : ( 195)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2017.00030.8

Development and Validation of RP-HPLC Method for simultaneous estimation of Telmisartan, Amlodipine Besylate and Hydrochlorthiazide in their tablet dosage form

Parmar Ashwini, Sonawane Sandeep*, Chhajed Santosh, Kshirsagar Sanjay

MET's Institute of Pharmacy, Bhujbal Knowledge City, Adgaon, Nashik-422 003, India

*Corresponding Author E-mail: sandeeps.iop@gmail.com

Online published on 17 October, 2017.


To treat the second stage high blood pressure, physicians prefers the combination drug therapy consisting of two or more anti-hypertensive agents. Among these, telmisartan with amlodipine besylate, telmisartan with hydrochlorthiazide as well as triple drug therapy consisting of amlodipine besylate, telmisartan and hydrochlorthiazide are commonly prescribed. In the present paper, a simple, accurate and precise RP-HPLC method was developed for the estimation of amlodipine besylate, telmisartan and hydrochlorthiazide in bulk and combined dosage form. All these three drugs were successfully separated and resolved from each other on C18 kinetex column (250 × 4.6 mm, 5 μ), using acetonitrile: 20mM phosphate buffer (pH 3.0) (60: 40%, v/v) as a mobile phase at a flow rate of 1 mL/min. The detection was performed at 258 nm. The developed method was further validated as per ICH Q2 (R1) guidelines.



Telmisartan, Amlodipine Besylate, validation. Hydrochlorthiazide, RP-HPLC, Analytical method.


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