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Asian Journal of Pharmaceutical Analysis
Year : 2017, Volume : 7, Issue : 3
First page : ( 185) Last page : ( 188)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2017.00029.1

Development and validation of RP-HPLC method for simultaneous estimation of ezetimibe and glimepiride in tablet dosage form

Parmar Ashwini, Sonawane Sandeep*, Chhajed Santosh, Kshirsagar Sanjay

MET's Institute of Pharmacy, Bhujbal Knowledge City, Adgaon, Nashik-42200, India

*Corresponding Author E-mail: sandeeps.iop@gmail.com

Online published on 17 October, 2017.

Abstract

A simple, accurate and precise RP-HPLC method was developed and validated for simultaneous estimation of glimepiride (GLM) and ezetimibe (EZE) in tablet dosage form. Both drugs were separated on C18 Phenomenex column (250 × 4.6 mm, 5 μ) using methanol: 20mM potassium phosphate buffer (pH 3.00) (80: 20% v/v) at flow rate of 1 mL/min. All eluents were detected at 230 nm. GLM and EZE were eluted at 5.292 and 4.183 min, respectively. The method was linear in the range of 2–10 μg/mL and 20–100 μg/mL for GLM and EZE, respectively. The developed method was further validated as per ICH Q2(R1) guidelines and applied successfully for the quantitation of tablet dosage form.

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Keywords

Ezetimibe and Glimepiride, RP-HPLC, Analytical Method Validation.

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