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Asian Journal of Pharmaceutical Analysis
Year : 2016, Volume : 6, Issue : 1
First page : ( 35) Last page : ( 40)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2016.00006.5

Zero order and Area under Curve Spectrophotometric Methods for Determination of Riboflavin in Pharmaceutical Formulation

Mali Audumbar1,*, Mali Seeta2, Bathe Ritesh1, Patil Manojkumar1, Tamboli Ashpak2

1Department of Pharmaceutics, Sahyadri College of Pharmacy, Methwade, Sangola-413307, Solapur, Maharashtra, India

2Department of Pharmaceutical Chemistry, Sahyadri College of Pharmacy, Methwade, Sangola-413307, Solapur, Maharashtra, India

*Corresponding Author E-mail: maliaudu442@gmail.com

Online published on 2 April, 2016.


Simple, fast and reliable spectrophotometric methods were developed for determination of Riboflavin in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the zero order derivative values measured at 445 nm and the area under the curve method values measured at 420-473nm (n=2). Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Riboflavin using 5–25μg/ml (r²=0.999 and r²=0.999) for zero order and area under the curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. Developed spectrophotometric methods in this study are simple, accurate, precise and sensitive to assay of Riboflavin in tablets.



Riboflavin, UV visible spectrophotometry, AUC, Method Validation, Zero order method.


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