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Development and Validation of Visible Spectrophotometric Method for Determination of Andrographolide in Kalmegh Plant Extract Suneetha A.*, Manasa K. Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Guntur, A.P-522002 *Corresponding Author E-mail: drasuneetha@gmail.com
Online published on 22 October, 2014. Abstract A simple, sensitive, precise and accurate UV Spectrophotometric method has been developed for estimation of andrographolide in kalmegh plant extract. The method is based on the formation of red-orange colored complex with picric acid in an alkaline medium and obeys beer's law in the range of 10–100μg/mL exhibiting the maximum absorbance at 481nm. The limit of detection and quantification were found to be 1.0μg/mL and 3.0μg/mL, respectively. The calibration curve demonstrated a linear relationship between the absorbance and concentration, with the correlation coefficient 0.9998. The Regression equation of the curve was Y=0.0114x + 0.0067. The method was validated according to ICH guidelines. Top Keywords Andrographolide, Picric acid, NaOH, Validation, ICH guidelines. Top | |
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