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Asian Journal of Pharmaceutical Analysis
Year : 2013, Volume : 3, Issue : 2
First page : ( 44) Last page : ( 47)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.

Novel Colorimetric Methods for quantitative estimation of Diacerin from capsule Formulation

Bais Sanjay1,*, Singhvi Indrajeet2, Chandewar Anil3, Shirsat Mrunal2

1Research Scholar, PRIST University, Thanjavur, Tamilnadu

2Pacific College of Pharmacy, Debari, Udaipur (Rajasthan) 313003

3P. Wadhwani College of Pharmacy, Yavatmal, Maharahtra

*Corresponding Author E-mail: sanjaybais@rediffmail.com

Online published on 19 June, 2014.


Two simple and sensitive colorimetric methods have been developed for the quantitative estimation of Diacerin from Pharmaceutical Capsule dosage form. Developed methods are based on the formation of chloroform extractable ion pair coloured complex of drug with Orange II (Method A) and Tropoelin (Method B). The complex formed in method A and B showed maximum absorbance at 435. Linearity was obeyed in concentration range of 50–350 μgm/ml and 50250 μgm/ml of Diacerin for method A and B respectively. The results of analysis were validated statistically and by recovery studies.



Diacerin, Orange II, Tropoelin, Colorimetric method.


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