A new simple RP-HPLC Method development, Validation and Forced degradation studies of Bilastine Mrs. Patel Khushbu K.*, Patel Arati M., Dr. Patel C. N. Department of Quality Assurance, Shri Sarvajanik Pharmacy College, Gujarat Technological University, Mehsana - 384001, Gujarat, India *Corresponding Author E-mail: khushbusspc@gmail.com
Online published on 1 October, 2021. Abstract A new simple, rapid, accurate and precise method for estimation of Bilastine in pharmaceutical dosage form by reverse phase liquid chromatography. The developed method employed mobile phase was Acetonitrile and Ammonium acetate pH 5.0 adjusted with glacial acetic acid with 85:15% v/v and flow rate 1.0ml/min. Method was developed using column C18 Water (150 × 4.6mm, 5μm) and detection wavelength was 215nm. The retention time was found to be 2.519 min. the proposed method was successfully applied to the determination of Bilastine in dosage form. High linearity of developed method was confirmed over concentration range of 25- 150 μg/ml and co-relation co-efficient is 0.996. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The recovery was in the range of 99 – 102% and limit of detection was found to be 0.45μg/ml and limit of quantification was found to be 1.20μg/ml. Bilastine was found to degrade under acid and oxidation conditions. There was no interference of excipient and degradation product in retention time so method was specific. Analytical parameter such as precision, accuracy, limit of detection, limit of quantification and robustness were determined according to international Conference on Harmonization (ICH) guidelines. Top Keywords Bilastine, RP-HPLC, Method Validation, Forced degradation study, Chromatography. Top |