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Asian Journal of Pharmaceutical Analysis
Year : 2021, Volume : 11, Issue : 1
First page : ( 41) Last page : ( 44)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2021.00008.9

Development and validation of rp-hplc method for the estimation of acyclovir in API and tablet formulation

Matole Vinod*, Dr. Thorat Yogesh, Javalgikar Akshay, Jamakhandi Shraddha, Pawar Pradip

D.S.T.S. Mandal’s College of Pharmacy, Solapur-413004, Maharashtra, India

*Corresponding Author E-mail: matole7414@gmail.com

Online published on 27 April, 2021.


The analytical method was developed and validated for determination of acyclovir in Tablet by High performance liquid chromatography. The separation was carried out on Luna C18 column (150*4.6mm, 5μm). The mobile phase consists of methanol: water in the ratio 50:50 at flow rate 1ml/min with diode array detector wavelength at 248 nm. The column temperature was adjusted at 38° ± 0.8±C with injection volume 20μl. The retention time of acyclovir was 2.718min. The linearity of the calibration curve was linear over the concentration range 40-60μg/ml (r2=1). The validation was carried out as per ICH guidelines. The development method was easy, rapid, linear, precise, accurate and consistent.



Acyclovir, RP-HPLC, Validation, Method development, 248 nm and Diode array Detector.


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