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Asian Journal of Pharmaceutical Analysis
Year : 2020, Volume : 10, Issue : 3
First page : ( 155) Last page : ( 157)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2020.00028.9

Development and validation of uv spectrophotometric method for estimation of ursodeoxycholic acid in bulk formulation

Patil Suraj J.*, Kane Sandeep R., Mohite Shrinivas K., Magdum Chandrakant S.

Department of Chemistry, Rajarambapu College of Pharmacy, Kasegaon-415404, Dist-Sangli, India

*Corresponding Author E-mail: patilsuraj0212@gmail.com

Online published on 16 September, 2020.


A rapid and economic UV spectrophotometric method developed by using a solvent methanol: acetonitrile (48:52) composed together to estimate the ursodeoxycholic acid content in bulk and pharmaceutical dosage formulation. Pre-determined study of λmax is 216nm it was proved linear range of 1.0–10.0μg/ml and exhibited good correlation coefficient (R2=0.992) and excellent mean recovery (98.00–100.05%). This method was successfully applied to the determination of λmax of ursodeoxycholic acid and results were in good agreement with label claims. The method was validated statistically and by recovery studies for linearity, precision, repeatability and reproducibility the obtained result proved that the method can be employed for the routine analysis of ursodexycholic acid in bulks formulation.



Ursodeoxycholic acid, UV Spectrophotometry, Method validation.


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