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Asian Journal of Pharmaceutical Analysis
Year : 2020, Volume : 10, Issue : 3
First page : ( 129) Last page : ( 133)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2020.00023.X

Zero-order derivative and area under curve uv-spectrophotometric methods for determination of calcium levofolinate in bulk and pharmaceutical dosage form

Rathod Rahul D.1, Nangare Sopan N.2,*, Patil Vikas R.1, Rathod Harshada S.1, Shitole Mayuri M.3, Tade Rahul S.2

1Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur-425405 Dist: Dhule (MS), India

2Department of Pharmaceutical Chemistry, H. R. Patel Institute of Pharmaceutical Education and Research, Shirpur-425405 Dist: Dhule (MS) India

3Research Scientist, Process Development Lab Department, Murli Krishna Pharma Pvt. Ltd., Ranjangaon-412209 Dist: Pune, (MS), India

*Corresponding Author E-mail: snangareopan@gmail.com

Online published on 16 September, 2020.


Simple, accurate, and precise UV-Spectrophotometric methods were developed for the estimation of Calcium levofolinate (CLF) in bulk and parenteral dosage form by Zero Order (Method A), Area Under Curve (AUC) (Method B). The solution of standard and sample was prepared in 0.1N sodium hydroxide (NaOH) and further dilution was carried out using Double Distilled Water (DDW). The quantitative determination of the drug was carried out by using the zero-order derivative values measured at 282±2nm (method A) and the AUC method values measured at 270.00nm - 297.80nm (method B). Calibration graphs constructed at their respective wavelength of determination were linear in the concentration range of CLF using 3–18μg/mL (r2=0.999) for zero-order and AUC spectrophotometric methods. Moreover, all these methods proposed were thoroughly validated with ICH guidelines. To summarize, developed spectrophotometric methods in this research are simple, precise, accurate, and sensitive to the assay of CLF in injections.



Calcium levofolinate, UV-spectrophotometer, Zero-order, Area Under Curve, Validation, ICH guidelines.


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