A Prospective Validation of Hydrocholorothiazide and Bisoprolol Fumerate in its Pure and Bulk Dosage Forms by using RP-HPLC Technique Beula S. Janet1,*, Dr. Suthakaran R.1, Bichala Prem Kumar1, Shankar CH.1, Ghouse Syed1, Suneetha K.2 1Vijaya College of Pharmacy, Munuganoor, RR District, India 2 SSJ College of Pharmacy, Gandipet, RR District, India *Corresponding Author E-mail: Pharmjanet123@gmail.com
Online published on 3 June, 2020. Abstract A simple, specific and accurate RP-HPLC method was developed for the simultaneous estimation of Bisoprolol fumerate and Hydrochlorothiazide in bulk dosage forms. The chromatographic conditions were successfully developed for the separation of Bisoprolol fumerate and Hydrochlorothiazide by using ACE C18 column (4.6×150mm) 5μ, flow rate was 1.2ml/min mobile phase ratio was (70: 30 v/v) methanol: Phosphate buffer pH 3 (pH was adjusted with orthophosphoric acid), detection wavelength was 240nm. The method was validated according to ICH guidelines for specificity, LOD, LOQ, Precision, Accuracy and Linearity. The method showed good reproducibility and recovery with % RSD less than 2. Top Keywords ACE C18 column, Hydrochlorothiazide and Bisoprolol, RP HPLC. Top |