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Asian Journal of Pharmaceutical Analysis
Year : 2020, Volume : 10, Issue : 1
First page : ( 1) Last page : ( 6)
Print ISSN : 2231-5667. Online ISSN : 2231-5675.
Article DOI : 10.5958/2231-5675.2020.00001.0

A Prospective Validation of Hydrocholorothiazide and Bisoprolol Fumerate in its Pure and Bulk Dosage Forms by using RP-HPLC Technique

Beula S. Janet1,*, Dr. Suthakaran R.1, Bichala Prem Kumar1, Shankar CH.1, Ghouse Syed1, Suneetha K.2

1Vijaya College of Pharmacy, Munuganoor, RR District, India

2 SSJ College of Pharmacy, Gandipet, RR District, India

*Corresponding Author E-mail: Pharmjanet123@gmail.com

Online published on 3 June, 2020.


A simple, specific and accurate RP-HPLC method was developed for the simultaneous estimation of Bisoprolol fumerate and Hydrochlorothiazide in bulk dosage forms. The chromatographic conditions were successfully developed for the separation of Bisoprolol fumerate and Hydrochlorothiazide by using ACE C18 column (4.6×150mm) 5μ, flow rate was 1.2ml/min mobile phase ratio was (70: 30 v/v) methanol: Phosphate buffer pH 3 (pH was adjusted with orthophosphoric acid), detection wavelength was 240nm. The method was validated according to ICH guidelines for specificity, LOD, LOQ, Precision, Accuracy and Linearity. The method showed good reproducibility and recovery with % RSD less than 2.



ACE C18 column, Hydrochlorothiazide and Bisoprolol, RP HPLC.


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