Developing PCR primers for identification of potato rot nemato (Ditylenchus destructor) in the Russian Federation and Iran Mahmoudi Niloufar1*, Pridannikov Mikhail2, Naserzadeh Yousef1, Pakina Elena1, Engeribo Albert1, Limantceva Liudmila3, Ivanov Anton3 1Department of AgrobiotechnologyInstitute of Agriculture, RUDN University, Moscow, 117198, Russia 2The Center of Parasitology, A.N. Severtsov Institute of Ecology and Evolution, RAS, Russia. 3All-Russian Plant Quarantine Center, 140150, Moscow region, Russia. *(e-mail: niloofarmahmoodi@ymail.com)
Online published on 25 January, 2021. Abstract The potato rot nematode (Ditylenchus destructor) species is an influential pathogenic potato nematode. Many countries impose quarantine restrictions on the presence of D. destructor in imported plant and soil materials. Therefore, the present investigation was conducted from 2018 to 2020 at the All-Russian Plant Quarantine Center of Russia Federation with samples collected from the Severtsov Institute of Ecology and Evolution, RAS, Russia and Iran. In the current study, we developed PCR with species-specific primers for the rapid and reliable separation of D. destructor using gel electrophoresis and melting curve analysis. A species-specific primer set was designed based on the nucleotide sequence of the gene. The PCR protocol was verified using samples of D. destructor and the closely related species, D. dipsaci and D. gigas. The species-specific primer set was able to detect D. destructor from samples containing mixtures of Ditylenchus species. The PCR species-specific protocol should allow for more rapid identification of Ditylenchus species recovered from plant materials than previously possible. Top Keywords Distribution, Ditylenchus destructor, Molecular identification, Plant parasitic nematode, Potato diseases. Top |