Department of Chemistry, Sri Satya Sai University of Technology & Medical Sciences, Sehore-466001, Madhya Pradesh, India
The purpose of this study is to develop simple, economic, specific, rapid, reliable, and reproducible method for simultaneous estimation of the Gabapentin, Mecobalamin and Alpha lipoic acid
In this method the overlapping spectra of Gabapentin, Mecobalamin and Alpha lipoic acid were well resolved by making use of the first-derivative of the ratios of their direct absorption spectra. The derivative ratio absorbance of Gabapentin, Mecobalamin and Alpha lipoic acid were measured at 731.10nm, 768.53 nm and 242.21nm for their quantification. The method was validated for accuracy, precision, linearity, robustness and sensitivity
Gabapentin, Mecobalamin and Alpha lipoic acid were shown linearity in the concentration range of 100e500 mg/ml, 0.5e2.5mg/ml and 100e500mg/ml respectively. The LOD and LOQ were found to be 3.09mg/ml and 9.37mg/ml; 0.03 mg/ml and 0.10mg/ml; and 4.79mg/ml and 14.52mg/ml respectively. The % labelled claim for Gabapentin, Mecobalamin and Alpha lipoic acid were found to be 98.71, 98.94 and 98.44 respectively
Thus, the described method is suitable for routine analysis and quality control of pharmaceutical preparations containing these drugs in combination.
Gabapentin, Mecobalamin, Reproducible method
