Department of Pharmaceutical Analysis, Gandhi Institute of Technology and Management, GITAM Institute of Pharmacy, Visakhapatnam, Andhra Pradesh-530045, India
New first derivative spectrophotometric methods have been established for the determination of Sitagliptin in pharmaceutical formulations. Sitagliptin is an oral dipeptidyl peptidase-4 inhibitor which exerts its actions in type 2 diabetes patients by slowing the inactivation of incretin hormones. Sitagliptin is chemically known as (R)4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro [1,2,4]triazolo [4,3-a] pyrazin -7(8H) -yl] -1-(2,4,5-trifluoro phenyl)butan-2-amine. First derivative spectrophotometric methods were developed using the reagents 0.1 N NaOH, phosphate buffers (pH 5.0 and 8.0), borate buffer (pH 9.0) and acetate buffer (pH 4.7) and amplitude was taken as the parameter from the derivative spectra to construct the calibration curve. Sitagliptin obeys BeerLambert's law over the concentration range 5–100 μg/ml in all the methods and the methods were validated as per ICH guidelines.
Sitagliptin, Derivative spectroscopy, Amplitude, Validation
