1Deptartment of Physiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, India.
2Department of Physiology, Shri Vinoba Bhave Civil Hospital Silvassa, Dadara and Nagar Haveli (UT)India, 396230.
3Deptartment of Oral and Maxillofacial Surgery, Manipal college of Dental Surgery, Mangalore, Manipal Academy of Higher Education, Manipal, KarnatakaIndia.
Objective: The Present study mainly focussed on the influential role of local neurotransmitter level in Orexin B induced Nucleus Accumbens (NAc) dependent voluntary ethanol consumption and preference. Methods: NAc in male Wistar rats were infused by Orexin B (low dose-3 nmol/μl and high dose 30nmol/μl) and Orexin B antagonist (TCS-OX2–29) (10μg/μl) sequentially. Food intake, alcohol intake, and two-bottle free choice preference tests (alcohol vs. water) were carried out at one hour, 2 hrs, 4 hrs, 12 hours, and 24 hrs in all the groups. Neurotransmitters level in the Nucleus Accumbens was also estimated at the end of the study. Results: Orexin B (30nm/μl) increased net alcohol intake in single-bottle test as well as in two-bottle free choice test. Whereas, Orexin B antagonist decreased alcohol consumption and preference to ethanol. Glutamate level significantly decreased and Dopamine and noradrenaline concomitantly increased. Conclusions: Orexin B plays a role in the modulation of ethanol consumption and preference confirmed by Orexin B antagonist infusion. The influence of neurotransmitter levels in Orexin B mediated ethanol consumption was also explored.
Alcohol, Glutamate, Nucleus Accumbens, Orexin B
