Research Journal of Pharmacy and Technology

SCOPUS
  • Year: 2019
  • Volume: 12
  • Issue: 9

Cytotoxic and Xanthine Oxidase Inhibitory Activities of Chemical Constituents of Alphonsea cylindrica King

  • Author:
  • Yuhanis Mhd Bakri1, Ahmed Kareem Obaid Aldulaimi1,, Saripah Salbiah Syed Abdul Azziz1,, Mohd Azlan Nafiah1, Saadon Abdulla Aowda2, Muhammad Hazim1, Khalijah Awang3, Yumi Zuhanis Has-Yun Hashim4, Phirdaous Abbas5
  • Total Page Count: 6
  • Page Number: 4129 to 4134

1Department of Chemistry, Faculty of Science and Mathematics, Sultan Idris Education University, 35900, Tanjong Malim, Perak, Malaysia

2Department of Chemistry, Faculty of Science, University Of Babylon, Babylon, Iraq

3Department of Chemistry, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

4International Institute for Halal Research and Training (INHART), International Islamic University Malaysia, P.O Box 10, 50728, Kuala Lumpur, Malaysia

5Department of Biotechnology Engineering, Kul. of Engineering, International Islamic University Malaysia, P.O Box 10, 50728, Kuala Lumpur, Malaysia

Abstract

This study aimed to investigate the chemical constituents from Alphonsea cylindrica as well as their cytotoxic and xanthine oxidase inhibitory activities. The chemical compounds were isolated and purified by various chromatographic techniques and their structures were elucidated via modern spectroscopic techniques including NMR, MS, IR, UV and comparison with literature. A study on the barks of A. cylindrica has found eleven known compounds were identified as stigmasterol (1), isoursuline (2), cyathocaline (3), kinabaline (4), muniranine (5), isooncodine (6), iraqiine (7), O-methylmoschatoline (8), kareemine (9), atherospermidine (10) and N-methylouregidione (11). The cytotoxic effect of hexane, DCM and methanol of bark of A. cylindrica as well as the isolated compounds; 1, 2, 3, 4, 5, 7, 8, 9 and 10 were estimated on MCF-7 human breast cancer cells and isoursuline possessed the most potent inhibitory activity with IC50 value of 33 μg/ml. Eight compounds 1, 3, 4, 5, 6, 8, 10 and 11 displayed moderate inhibitory activity effect with IC50 < 100 μM against xanthine oxidase. As a conclusion, eleven compounds have been isolated from the bark of A. cylindrica King. Some of the compounds also showed potential against cytotoxicity and xanthine oxidase inhibitory activities. The findings of this study can enhance the understanding of the chemotaxonomy aspect of the A. cylindrica as well as its potential in traditional and modern medicine.

Keywords

Alphonsea cylindrica, Alkaloid, Cytotoxic, MCF-7, Xanthine Oxidase