Research Journal of Pharmacy and Technology

Uropathogenic Escherichia coli (UPEC) is characterized by complex natures with ceaselessly developing for virulent from non-virulent isolates cause strong illness. Vaccines against UPEC are not widely available, leading to exceeding the incidence of these isolates impervious for the antimicrobial agent. This requires constant efforts to control diseases and to know the development of infection and immune response. After infection and immunization, ninety clinical isolates identified as UPEC were obtained from hospitalized patients from three hospitals in south Iraq and identified by the biochemical test. All isolates were tested for susceptibility testing. One isolate was chosen for the immunization and vaccine model, the one remittent for most antibiotics. ELISA was used to assessments of immunoglobulin (IgG) level to serum mouse at 14, 28 and 42 days of immunization. Results proved that isolate showed resistance to all antibiotics. There are highly significant differences when using UPEC in the level of IgG reaches the peak ranged (685.3 ±49.8) at 28 days as compared with control for assessment humoral immune response. Also, high significant differences thickness of the footpad skin reaches (5.021±0.210) after 24 hours of the immunized mice, so that vaccine with this isolate has a potential for development as a candidate vaccine for protection against infection.