1Department of Preventive and Social Dentistry, School of Dentistry, Kyung Hee University, Hoegi-dong, Dongdaemoon-gu, Seoul, 130–701, Republic of Korea
2Department of Dental Hygiene, Hanseo University, 360 Daegok-Ri, Haemi-Myun, Seosan-Si, Chungnam, 356–706, Republic of Korea
The purpose of this research was to determine a method to analyze several salivary anions simultaneously using HPLC (IC) and to analyze the anions in actual saliva samples.
Chromatographic determination was performed using a Dionex Ion column Pac AG 11-HC 50mm×4mm. The column parameters were as follows: mobile phase, isocratic elution with 120 mmol/L NaOH; flow rate, 1, 000 μL/min; separation temperature, 35°C.
The linearity of the sample's linear range was 0.9902–0.9989, LOD was 0.78–1.16μM, and LOQ was 2.36–3.49 μM. The recovery test showed a recovery rate of 95.63–107.77%, and RSD of 3.37–11.48%, verifying the method of analysis.
The method of analysis used in this study can appropriately quantify anions in saliva. Hence, it could be used in biomarker studies.
Saliva, Inorganic anion, Ion, Biomarker, HPLC
