Research Journal of Pharmacology and Pharmacodynamics

In the present study, the ehanolic leaf extract of Azima tetracantha Lam. (A. tetracantha) was investigated for analgesic activity in mice using hot plate method. The leaves of A. tetracantha were collected from Ponnamaravathi and cut into small pieces and shade dried. The dried powdered leaves (100 gm) were extracted in a Soxhlet apparatus by using 95% ethanol. Mice weighing 15–25 gm were taken for the experiment, divided into four groups of six animals each. Group 1 received the (normal saline 2 ml/kg) group 2 and group 3 received ethanolic leaf extract of A. tetracantha 100 and 200 mg/kg respectively and the group 4 served as standard (Pentazocine 5 mg/kg) by oral route. Each mouse was picked on the hot plate, which was maintained at constant temperature 55°C±0.05. The basal reaction time, the time taken for the jump response (or) paw licking which ever appears to be first was observed. The reaction time of animals in all groups was noted at 0, 30, 60, 120 and 180 seconds after the administration of the drug. All data were analyzed with student ‘t’ test. A maximum analgesic activity was found at 120 sec, after drug administration, which was compared to the standard drug used as Pentazocine (5 mg/kg).