In vitro regeneration of ‘Brahmi ’[Bacopa monnieri (L.) Pennel] and preliminary analysis of its secondary metabolites
Abstract
A standard protocol for in vitro regeneration of Bacopa monnieri has been successfully developed. Best shoot initiation and multiple shooting were obtained on BAP 5mg L−1 from leaf explants, whereas liquid basal medium produced maximum number of roots. In vitro regenerated plants were successfully hardened and about 90% plants survived on field transfer. The saponin content in both the nature grown and in vitro raised plants were analyzed. In vitro raised plants were found to have low saponin content.
Keywords
Bacopa monnieri, in vitro shoots, micropropagation, rooted plantlets, saponins
