Indian Journal of Plant Genetic Resources

The pummelo (Citrus grandis Merr.), the largest fruit size in citrus species, is an underutilized fruit in India. Pummelo in Bihar is mostly grown as homesteads and this fruit is to be developed as potential table fruit because of thick rind, easy to handle and transport. Genetic diversity is an important factor in any crop improvement programme for obtaining high yielding cultivars. Variation among seedling progenies is a rule in pummelo since it is monoembryonic Citrus spp. This study was conducted through four cell analysis in the five project communities under the UNEP-GEF project at the Pusa Site, Bihar during 2012 with aim for estimation of genetic diversity for physico-chemical traits, selection of superior clones and to draw the interrelationships between observations for precise characterization of pummelo seedling. Superior plants were also identified based on the highest yield i.e. number of fruits/plant (> 300). Statistical analysis for physico-chemical parameters revealed significant differences among the selected plants for the 12 horticultural traits indicating the higher variability. Principal Component Analysis (PCA) and cluster dendrogram performed on the basis of studied parameters showed continuous variation in the fruit physico-chemical traits and many groups were formed, indicating existence of large and continuous variation. The first four PCs contributed 68.30% of the variability for fruit quality and PC1 accounted for 30.44% (acidity percentage) followed by fruit length (PC2), fruit width (PC3) etc. The maximum coefficient of variation was found in in case of rind thickness, 100 seed weight and acidity percentage. A large number of variability of pummelo with seedling origin and monoembryonic helps in identification of 4 pummelo clones for pulp colour (2 each for dark red flesh and pink flesh) with excellent eating quality, 9 superior clones were identified for profuse bearing with red fleshed and early. The above variation in the selected pummelo seedlings can be exploited for development of superior varieties, marketing and maintenance of exiting superior clone and above all grower can be ensured livelihood security through homestead farming practices in the community.