Indian Journal of Nematology

SCOPUS
  • Year: 2014
  • Volume: 44
  • Issue: 1

Quantitative Differences in Gene Expression of Defence Genes in Solanum tuberosum and S. sisymbriifolium Infected with Globodera pallida

  • Author:
  • Ayano Sasaki Crawleya,c,, Katarzyna Dybala, Stephen J. Powersa, Michael Birketta, John Picketta, David Nelsona, Rod Blackshawc, Renato Carvalhoa,d, Rosane Curtisa,e,
  • Total Page Count: 11
  • DOI:
  • Page Number: 62 to 72

aRothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK

cCentre for Agricultural and Rural Sustainability, Plymouth University, Drake Circus, Plymouth, Devon PL48AA, UK

dMonsanto do Brasil, Sao Paulo, Brazil, 04578-910

eBionemax UK Limited, Rothamsted Centre for Research and Enterprise, Harpenden, Hertfordshire AL5 2JQ, UK

Abstract

Solanum sisymbriifolium is an effective trap crop for the potato cyst nematodes, Globodera pallida and G. rostochiensis, with strong hatching ability and total immunity to both species. To investigate the mechanism of this immunity, expression changes of defence-related genes were studied in roots infected by G. pallida and compared with those in roots of potato, S. tuberosum. The results revealed that the potato basic class I chitinase gene was up-regulated at 3 days post inoculation in S. sisymbriifolium but not in S. tuberosum, indicating that the chitinase gene is a hallmark for the immunity of S. sisymbriifolium to G. pallida.

Keywords

Potato cyst nematode, Globodera pallida, Globodera rostochiensis, Trap crop, Solanum tuberosum, Solanum sisymbriifolium, Immunity, Defence-related gene, Chitinase gene