Indian Internet Journal of Forensic Medicine & Toxicology

  • Year: 2003
  • Volume: 1
  • Issue: 2

Rapid determination of carboxyhemoglobin in blood by oximeter

  • Author:
  • C Brehmer, P X Iten
  • Total Page Count: 1
  • Published Online: Jun 1, 2003
  • DOI:
  • Page Number: 1 to 1

Abstract

Different methods to determine carboxyhemoglobin(COHb) in blood are described in the literature. In our laboratory three methods to analyze COHb in post-mortom blood samples were compared: the spectrophotometric method of Maehly, a gas chromatographic metod with a theral conductivity detectoe (GC-TCD) and the Oximeter.Several COHb containing blood samples of deceased persons were analyzed.Results of all three methods were caomparable for low concentrations (ca.10% COHb) aewell asfor high concentrations (ca.80% COHb) regardless of the viscosity of the blood samples. The advantages of the Oximeter when compared to aehly's ethod and GC-TCD are extreme short time of analysis (<1min>.very small blood volue required (<0.1 ml) and easy handling. In our opinion application of Oximeter is not limited to analyses of blood samples fro living persons (e.g. in clinical toxicology): it can aswell be used for the deterination of COHb in postmortem blood samples. Hence it is a useful and time saving tool in forensic toxicology