The groundnut samples with peanut bud necrosis were collected from different parts of north eastern Karnataka and subjected to DAC-ELISA technique using polyclonal antiserum of PBNV and TSWV. All samples reacted positively with PBNV, while gave negative reaction against TSWV antisera. The weed samples prevailing in groundnut ecosystem, subjected to DAC-ELISA against PBNV also gave negative reaction. ELISA for different symptomatic PBND groundnut plants revealed that the primary symptomatic plants contained higher virus concentration. Furthermore, PBNV infected leaves with primary symptoms had highest virus titre. The study revealed that the virus causing bud necrosis in groundnut and tomato in north eastern Karnataka was PBNV. There was no natural infection of PBNV in major weeds prevailing in groundnut fields.
Peanut bud necrosis disease, groundnut, ELISA
