Asian Journal of Research In Chemistry

  • Year: 2014
  • Volume: 7
  • Issue: 7

Optimization of Enzymatic Synthesis of 3-O-β-D-Glucopyranoside Betulinic Acid by Novozyme-435

  • Author:
  • Hamisu Abdu1,3, Faujan B. H. Ahmad2,, M. Basri1, Intan Safinar Ismail1, M.B. Abdul Rahman1
  • Total Page Count: 4
  • DOI:
  • Page Number: 640 to 643

1Department of Chemistry, Faculty of Science, University Putra Malaysia, Malaysia

3Hussaini Adamu Federal Polytechnic, Kazaure, Jigawa State, Nigeria

2Natural Product Laboratory, Faculty of Applied Sciences, Universiti Teknologi MARA, Malaysia

Abstract

The enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid using Novozyme-435 as a catalyst was studied. The effects of various parameters such as reaction temperature, reaction time and amount of enzymes were investigated. The optimum reaction conditions for the synthesis of 3-O-β-D-glucopyranoside betulinic acid was obtained using 30.67 h, 54.30°C and 180 mg of enzymes using betulinic acid (0.05 mmol) and glucose (0. 1 mmol) respectively. The actual percentage yield under these conditions was 88.69% and the predicted value was 89.05%.

Keywords

Enzymatic synthesis, 3-O-β-D-glucopyranoside betulinic acid, Novozyme-435 and optimization