Isolation of Apigenin From Trigonella foenum Graecum Seed Extract Using Column Chromatograpy on Lab Scale
Abstract
Methanolic extract of Trigonella foenum graecum seeds contains flavonoids such as rutin and Apigenin as major constituent. Column chromatographic technique was used as attempt on lab scale for separation of Apigenin by using butanol: ethyl acetate (1: 1) as mobile phase. The compound isolated from column was evaluated by performing HPTLC, UV, IR and HPLC with standard Apigenin. This method showed an excellent performance in separating the flavonoid Apigenin
Keywords
Column chromatography, Trigonella foenum graecum, Apigenin
