1Shri Adichunchunagiri College of Pharmacy, B. G. Nagara, Tal: Nagmangala Dist: Mandya, Karnataka, India
2Government College of Pharmacy, Vidyanagar, Karad, Dist-Satara, Maharashtra-415 124, India
Simple, precise, sensitive and reproducible quantitative reverse phase HPLC method has been developed for estimation of Sitagliptin phosphate in pharmaceutical dosage form. The method was carried out on a Phenomenex RP 18 (2) Column (250×4.6mm i.d; particle size 5 μm) column with a mobile phase consisting of (60:40 v/v Acetonitrile: water) at a flow rate of 1.0 ml/min. The eluents were monitored at 272 nm. The retention time of Sitagliptin phosphate was found out to be 5.062. The developed method was validated in terms of accuracy, precision, linearity, limit of detection and limit of quantification. The proposed method can be used for the estimation of this drug in pharmaceutical dosage forms.
Sitagliptin phosphate, Validation, Estimation
