ASIAN JOURNAL OF RESEARCH IN CHEMISTRY

  • Year: 2017
  • Volume: 10
  • Issue: 3

Effect of osmotic stress on l-glutamic acid production by an auxotrophic mutant Micrococcus glutamicus AB100

1Vidyasagar College, Main Campus, 39 Sankar Ghose Lane, Kolkata, 700 006

2Department of Chemical Engineering, Biochemical Engineering Subdivision, Biotechnology Laboratory, A.P.C Road, Kolkata-700009

Abstract

An experimental study was carried out to examine the effect of osmotic stress on L-glutamic acid production by the mutant Micrococcus glutamicus AB100. Initial addition of NaCl resulted in an arrest of the bacterial cell growth and glucose consumption. The cell growth was found to resume within few hours. Maximum cell growth was obtained with 1.0 (M) NaCl. Micrococcus glutamicus AB100 was found to grow even with 2.0(M) -3.0(M) NaCl.

Keywords

Experimental, L-glutamic acid, Micrococcus glutamicus AB100, NaCl]