Asian Journal of Pharmaceutical Analysis

High performance liquid chromatography (HPLC) is an important qualitative and quantitative technique, mostly used for the determination of pharmaceutical and biological samples. It is the most versatile, safest, dependable and useful chromatographic technique for the quality control of drug components. This article was prepared with different trends of HPLC, such as principle, types, instrumentation and application. It represents convenient new trends in HPLC such as RRLC, UPLC, UFLC and Nano LC. New techniques have mainly increased the resolution power for complex sample analysis.