Bait Techniques for Isolation of Pythium aphanidermatum Causing Damping-off of Chilli from Soil and Efficacy of Bio-agents In Vitro Patel J. K.1,*, Joshi K. R.2, Prajapati H. N.1, Jage N. P.2 1Department of Plant Pathology, B. A. College of Agriculture, AAU, Anand-388 110 2Department of Plant Pathology, Bidi Tobacco Research Station, AAU, Anand 388 110 *Email: jignesh0204@gmail.com
Online published on 24 December, 2014. Abstract A French bean/bottle gourd wasused as a bait. A bait fruit was transversely cut into 2–4 piece and buried into the infested soil at a depth of 5–6 cm below the soil surface. The soil was irrigated and kept moist. After 24–48 hour observed mycelial growth aseptically transferred to potato dextrose agar media. Eight knownbio agents evaluated by two different methodviz., Pathogen at centre and dual culture techniques. Among them the Trichoderma harzianum and Bacillus subtilis were most effective antagonist in both methods. Maximum inhibition of mycelial growth of the pathogen by dual culture method was obtained with Trichoderma harzianum (38.89%). The next best antagonist in order of percent growth inhibition was Bacillus subtilis (32.64%). While in pathogen at centre method bacterial bioagents Bacillus subtilis (75.69%) was inhibitory than fungal bioagent Trichoderma sp. Top Keywords Pythium, Isolation techniques, Soil, Bio-agent. Top |