In Vitro Antibacterial Activity of Leaf Extracts of Hibiscus Syriacus (L) Punasiya Rakesh1,*, Pillai Sujit2 1Department of Pharmaceutical Biotechnology, GRY Institute of Pharmacy, Borawan, Khargone-451228, India 2Department of Pharmaceutical Chemistry, GRY Institute of Pharmacy, Borawan, Khargone, MP *Corresponding Author E-mail: rakeshpunasiya@yahoo.com
Online published on 30 July, 2015. Abstract The antimicrobial activity of different crude extract like petroleum ether, benzene, chloroform, methanol, and aqueous of Hibiscus syriacus L. plants used in traditional Indian medicine was tested by well diffusion method against four bacterial pathogens: Bacillus cereus (MTCC-430), Staphylococcus epidermis (MTCC-435), Klebsiella pneumonia (MTCC-432), and Bacillus subtilis (MTCC 121) bacteria. The minimum inhibitory concentration (MIC) was determined for evaluating the potential leaves extract. The antibacterial activity of methanol extracts (0.5 g/ml) of Hibiscus syriacus L. showed maximum zone of inhibition (19.1 mm, 19.6 mm, 22.9 mm and 25.4 mm, respectively) against Bacillus cereus (MTCC-430), Staphylococcus epidermis (MTCC-435), Klebsiella pneumonia (MTCC-432), and Bacillus subtilis (MTCC 121) bacteria. MIC was tested at various concentrations from 0.625 mg/ml to 100 mg/ml for all the leaves extracts. The Klebsiella spp was the most resistant strain and various concentrations of all the leaves extract benzene extract showed less activity against the tested microorganism. The present screening result demonstrated that the methanol leaves extract of plants Hibiscus syriacus L. has potent antibacterial activity and the studied plants may be new source for novel antibacterial. Top Keywords Antibacterial activity, Well diffusion method, Zone of inhibition, Pathogens. Top |