(18.97.14.85)
[ij] [ij] [ij] 
Email id
 

Research Journal of Pharmacy and Technology
Year : 2014, Volume : 7, Issue : 6
First page : ( 638) Last page : ( 642)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.

Development of UV Spectrophotometric method for the analysis of Antipsychotic drug

Yasir Mohd1,2,*, Sara UVS3

1Department of Pharmacy, Uttarakhand Technical University, Dehradun-248007 (Uttarakhand), India

2Department of Pharmaceutics, ITS Pharmacy College, Delhi –Meerut Highway, Muradnagar, Ghaziabad – 201206 (UP), India

3College of Pharmaceutical Sciences, RKGIT, Delhi –Meerut Highway, Muradnagar, Ghaziabad – 201206 (UP), India

*Corresponding Author E-mail: mohdyasir31@gmail.com

Online published on 29 August, 2014.

Abstract

The aim of the present work was to develop and validate a sensitive, simple, accurate, precise and cost effective UV spectrophotometric method for the analysis of antipsychotic drug i.e haloperidol in prepared pharmaceutical formulations of solid lipid nanoparticles. The different analytical performance parameters such as linearity, range, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ) were determined according to International Conference on Harmonization (ICH) Q2 (R1) guidelines. The study was performed in phosphate buffer of pH 7.4. The peak (λmax) of haloperidol appeared at a wavelength of 247.5 nm in Phosphate buffer (pH 7.4). BeerLambert's law was obeyed in the concentration range of 2–20 μg/ml with correlation coefficient (R2) 0.9994. The results of the study demonstrated that the developed procedure is accurate, precise and reproducible (relative standard deviation <2%), while being simple, cheap and less time consuming. The results of stability study indicate that the drug was stable in selected medium after storage up to 48 h and was not affected by light and dark cycles. Therefore, this method can be suitably applied for the estimation of haloperidol in prepared solid lipid nanoparticles.

Top

Keywords

Antipsychotic drug, haloperidol, stability, UV spectrophotometric method, validation.

Top

  
║ Site map ║ Privacy Policy ║ Copyright ║ Terms & Conditions ║ Page Rank Tool
861,031,950 visitor(s) since 30th May, 2005.
All rights reserved. Site designed and maintained by DIVA ENTERPRISES PVT. LTD..
Note: Please use Internet Explorer (6.0 or above). Some functionalities may not work in other browsers.