Validated RP-HPLC Method for the Quantitation of Candesartan in Bulk and Pharmaceutical Dosage Forms. Kulsum Syeda1,*, Sagar G. Vidya2, Padmalatha M.1 1Department of Pharmaceutical Analysis, Vijaya College of Pharmacy, Munaganoor (V), Hyathnagar (M), Hyderabad, India-501505 2KSKV Kachchh University, Bhuj, Kutch, Gujarat, India *Corresponding Author E-mail: syedakulsum@gmail.com
Online published on 3 April, 2013. Abstract A simple, specific, accurate, precise and sensitive Reverse Phase High Performance Liquid Chromatographic method has been developed for the quantitation of candesartan in both pure and pharmaceutical dosage forms. An Inertsil ODS-3V C-18, 5 um column having 250 x 4.6 mm internal diameter in isocratic mode with mobile phase containing ortho phosphoric acid: Acetonitrile (20:80v/v) and adjust the pH to 3.2 by using 0.03M potassium hydrogen phosphate buffer. The flow rate was 1.0 ml/min and the effluents were monitored at 210 nm. The retention time was 8.057min. The linearity was in the range of 50–160 μ g/ml. This method was validated for linearity, precision, limit of detection, limit of quantitation and accuracy. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug. Top Keywords RP-HPLC, candesartan, Validation, mobile phase, inertsil column. Top |