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Development of RP-HPLC Method for Estimation Glipizide Karpillai Mousumi, Dhangar Sachin* G.R.Y. Institute of Pharmacy, Borawan, Khargone-451001, Madhya Pradesh, India *Corresponding Author E-mail: dhangar_saachin@rediffmail.com
Online published on 3 April, 2013. Abstract A reversed phase HPLC method is developed for the determination of glipizide in pharmaceutical dosage form. Chromatography was carried out on an inertsil C18 column using a mixture of methanol and phosphate buffer (pH 3.4) (55:45 v/v) as the mobile phase at a flow rate of 1ml/min. Detection was carried out at 225nm. The retention time of the drug was 7.098min. The method produced linear responses in the concentration range of 15–75 μg/ml of Glipizide. The method was found to be applicable for the determination of the drug in tablets. Top Keywords Glipizide, Estimation, Tablets, RP-HPLC. Top | |
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