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RP-HPLC Determination of Olmesartan Medoxomil and Amlodipine Besylate in tablets Priyadharisini J., Saraswathi D., Aruna Ajitha, Suresh A. Jerad* Department of Pharmaceutical chemistry, Madras Medical College, Chennai-600003 *Corresponding Author E-mail: ajsuresh2001@yahoo.co.uk
Online published on 3 April, 2013. Abstract A reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed for the estimation of Olmesartan Medoxomil and Amlodipine Besylate in bulk and pharmaceutical formulations. The quantification was carried out using RP-C -18 Phenomenex (250x4.6mm) in isocratic mode with mobile phase comprising50mM Ammonium acetate and Acetonitrile (50:50) v/v. The mobile phase was pumped at a rate of 1.0mL/min and detection was carried out at 239nm.The linearity was found to be in the range 25–125μg/mL. The proposed method was statistically evaluated and can be applied for routine quality control analysis of Olmesartan Medoxomil and Amlodipine Besylate. Top Keywords Olmesartan medoxomil, Amlodipine besylate, HPLC, Tablets. Top | |
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