Visible Spectrophotometric Determination of Abacavir Sulphate in Bulk Drug and Tablet Dosage Form Rao C.H. Prasada1,*, Rao J.V.L.N. Seshagiri2, Dhachinamoorthi D.1, Lakshmi Aswini G.3, K. Ashok1 1Dept. of Pharmaceutical Analysis, QIS College of Pharmacy, Ongole -523272, India 2Dept. of Pharmaceutical Analysis, University College of Pharmaceutical sciences, Andhra University, Vishakapatnam -530003, India 3Dept. of Pharmaceutical Analysis, Vagdevi College of Pharmacy, Gurazala -522415, India *Corresponding Author E-mail: prasadpharmach@gmail.com
Online published on 3 April, 2013. Abstract A simple visible spectrophotometric method has been developed for the estimation of abacavir sulphate in bulk and tablet dosage form. This method is based on the diazotization of abacavir sulphate with nitrous acid to form diazotized abacavir sulphate, followed by its coupling with β-naphthol to form a red coloured chromogen which shows maximum absorption at 574.0nm and obeys Beer's law in the concentration range of 5-20mcg/ml. This method was validated for precision, accuracy, ruggedness and robustness. Statistical analysis proves that the method is reproducible and selective for the estimation of said drug. Top Keywords Visible spectrophotometry, Abacavir sulphate, β-naphthol, Validation. Top |