Simultaneous Determination of Nebivolol and Amlodipine in Combined Dosage Form by Derivative Spectrophotometry Patel Samixa R.1,*, Patel Shweta M.2, Patel Jignesh I.3, Patel Paresh U.2 1Atmiya Institute of Pharmacy, Department of Pharmaceutical Chemistry, ‘Yogidham Gurukul’, Kalawad Road, Rajkot-384005, Gujrat, India 2S.K. Patel Colleges of Pharmaceutical Education and Research, Ganpat Vidyanagar, Kherva, Mehsana-382711, Gujarat, India 3B K Modi Govt Pharmacy Colleges, Polytechnic Campus, Bhavnagar Road, Rajkot-360 003 *Corresponding Author E-mail: samixa.patel@gmail.com, samixa.patel@yahoo.co.in
Online published on 3 April, 2013. Abstract Simple and accurate method to determine Nebivolol and Amlodipine, in tablet dosage form, was developed and validated using Derivative Spectrophotometry. Derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first-order derivative spectra were obtained at N = 1 (scaling factor), δλ = 2.0 nm, and determination was performed at 226.5 nm (ZCP of Amlodipine) for Nebivolol and 245 nm (ZCP of Nebivolol) for Amlodipine over the concentration range of 10–70 μg/mL for both drugs with mean recovery of 100.2 ± 1.25% and 100.1 ± 1.38% for Nebivolol and Amlodipine, respectively. Method was validated, and the results were compared statistically. They were found to be simple, sensitive, accurate, precise, reproducible and economical. The method was successfully applied for the determination of Nebivolol and Amlodipine in tablet dosage form without any interference from common excipients. Top Keywords Nebivolol, Amlodipine, Derivative spectrophotometry, zero crossing point. Top |