Determination and Pharmacokinetics of Ibuprofen in Rabbit Plasma by GC-MS Method Yilmaz Bilal1,*, Akba Vedat2 1Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey 2Criminal Police Laboratory, 25060, Erzurum, Turkey *Corresponding Author E-mail: yilmazb@atauni.edu.tr
Online published on 3 April, 2013. Abstract This paper describes a gas chromatography-mass spectrometry (GC-MS) method for determination of ibuprofen in rabbit plasma. Ibuprofen and internal standard (IS) naproxen were extracted from plasma by using liquid-liquid extraction method. The samples were separated by GC on a DB-5MS analytical column and determined by a quadrupole mass spectrometer detector operated under selected ion monitoring mode (SIM). Excellent linearity was found between 0.05 and 5.0 μg/mL (r=0.998) for plasma samples. Intra-day and inter-day precisions expressed as the relative standard deviation (RSD) for the method were 3.29–5.14% and 4.26–5.84%, respectively. The mean recovery of ibuprofen samples was 92.03%. This assay was successfully applied to a pharmacokinetic study of ibuprofen in New Zealand white rabbits. As a result, the plasma half-life was 2.68 ± 0.920 h and the mean AUC0–12 h was 187.24 ± 22.42 μg/mL h. The maximum plasma concentration (Cmax) of 59.8 ± 7.416 μg/mL reached 2.0 ± 0.288 h after administration. Top Keywords Ibuprofen, GC-MS, Liquid-liquid extraction, Pharmacokinetics. Top |