Ceropegia rupicola, Acokanthera schimperi, Jatropha variegate Antimicrobial, cytotoxicity and phytochemical Elkhateeb Waill A.1, El-Ghwas Dina E.1,*, Ahmed Soliman A. F.2, Elnahas Marwa O.1, Daba Ghoson M.1 1Chemistry of Natural and Microbial products DepartmentPharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt 2Department of Pharmacognosy, Pharmaceutical and Drug Institute, National Research Centre, Dokki, Giza, 12622, Egypt *Corresponding Author E-mail: dinaelghwas7781@yahoo.com, de.el-ghwas@nrc.sc.eg
Online Published on 13 October, 2023. Abstract In Asian countries, a variety of medicinal plants are utilized in folk medicine for hemostatic and antiseptic. The methanolic extracts of Jatropha variegata, Acokanthera schimperi, and Ceropegia rupicolawere tested for the following purposes: first, isolation of endophytic fungi, second, antimicrobial and cytotoxicity activity, and third, phytoconstituents of the methanolic extract using the GC-Mass technique. The plants came from a variety of Yemeni locations. Endophytic fungus strains were isolated and identified using starch casein and potato dextrose agar medium. Methanol was used to extract the stem and leaves of the plants. The agar well diffusion method was used to test the antibacterial properties of the produced methanolic extracts against four Gram-negative bacteria, three Gram-positive bacteria, and one species of yeast. The IC50 values for the most promising extracts are calculated. The methanolic extracts were also subjected to a phytochemical screening by GC-Mass. A total of forty fungal endophyte isolates were isolated. Fusarium spp. (15), Phomaspp. (6), Alternaria alternata (5), White Sterile mycelia (6), Dark Sterile mycelia (6), Cochlioboluslunatus (1), and Mucor spp. (1). were found throughout the identification process. The highest yield extract was obtained from Jatropha variegate (45.4%), followed by Acokanthera schimperi (32.3%), and finally Ceropegia rupicola (20.5%). Only Gram-negative bacteria were found to have antibacterial activity, and the most effective extract was Acokanthera schimperi against the majority of microorganisms tested (8-18mm). The methanolic extracts of Jatropha variegate had the best antibacterial activity against Klebsiella pneumoniae ATCC 13883015, with a zone of inhibition (45 mm). Furthermore, Ceropegia rupicola extract had the highest inhibitory zone against Candida albicans ATCC 700012 (20mm). In addition, the IC50 results showed that the Jatropha variegate extract on A549 had an IC50 of 84.1ug/ml and a PC3 of 39.4ug/ml. The IC50 for Ceropegia rupicola extract was 49.4ug/ml in A549 cells and 34ug/ml in PC3 cells. Also, the screening for phytochemicals of the three plant extracts revealed the presence of many chemicals such as ethanol, formic acid, and others that may be responsible for the observed actions. Top Keywords Acokanthera schimperi, Antimicrobial, Ceropegia rupicola, Jatropha variegate, GC-mass. Top |