In vitro Assessment of the antidiabetic activity of aqueous and ethanolic extracts from the aerial parts of Ajuga orientalis L. Althaher Arwa R.* Department of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman11733, Jordan *Corresponding Author E-mail: a.althaher@zuj.edu.jo, a.althaher@outlook.com
Online Published on 13 October, 2023. Abstract Ajuga orientalis L. is a member of the Lamiaceae family. Many biological properties of A. orientalis, such as antibacterial, anticancer, antioxidant, and anti-inflammatory activities, have been documented. The current study aims to assess the in vitro antidiabetic efficacy of aerial parts A. orientalis extracts through digestive enzymes inhibition assay (α-amylase and α-glucosidase), which are responsible for the digestion of poly and oligosaccharides. Acarbose, aqueous, and ethanolic extracts of A. orientalis were utilized in various concentrations (100, 200, 300, 400, and 500μg/ml). The absorbance values for the enzymes α-amylase and α-glucosidase at 540nm and 400nm, respectively, were measured using a spectrophotometer. Both extracts demonstrated significant inhibition of α-amylase and α-glucosidase enzymes in a dose-dependent manner. Furthermore, the ethanolic extract showed more inhibitory activity than the aqueous extract. In conclusion, A. orientalis extracts exhibited in vitro antidiabetic activity. Top Keywords Ajuga, Lamiaceae, Antihyperglycemic Agent, Alpha-Glucosidases, Alpha-Amylases. Top |