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Research Journal of Pharmacy and Technology
Year : 2023, Volume : 16, Issue : 4
First page : ( 1709) Last page : ( 1713)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.52711/0974-360X.2023.00281

Effect of omega 7 on some parameters related to WBCs and RBCs in methotrexate-induce blood toxicity in mice

Hassani Zahraa Hilal1,*, Hassan Ali Faris2

1Ministry of Health and Environment, Wasit Health DepartmentWasit, Iraq

2Universities of Baghdad, College of Pharmacy, Pharmacology and Toxicology Department

*Corresponding Author E-mail: zahraa.hilal1989@gmail.com

Online Published on 13 October, 2023.

Abstract

Background

Although methotrexate (MTX) is a frequently used chemotherapy drug, its effectiveness is sometimes hampered by the drug's toxic consequences. Omega 7 is a monounsaturated fatty acid, with different anti-inflammatory, anti-diabetic anti-obesity applications, and its possible protective effects against MTX-induced blood toxicity were investigated in this study.

Objective

Evaluation of possible protective effects of omega7 against MTX-induced blood toxicity.

Methods

30 mice were divided into five groups, the First group take liquid paraffin orallyfor 7 days for served as negative control and the second group take methotrexate (20mg/kg) intraperitoneallyto serve as a positive control,the third group takes omega 7 (100mg/kg)orally for 7 days, forth groupreceived (50mg/kg ) omega 7orally for 7 days as well as give It methotrexate (20mg/kg) on day 8, the fifth groupreceived (100mg/kg ) omega 7orally for 7 days as well as give It methotrexate (20mg/kg) on day 8. After that, the animals were killed and took blood samples for measuring blood parameters, such as PCV, Hb, MCV, MCH, platelet, WBC count and Differential WBC.

Results

The results showed the presence of a decrease in both the RBC from which MCV and MCH count showed a significant decrease at a dose of 20mg/kg concentration of methotrexate, omega7 at a dose of 50 and 100 mg/kg work to increase the variables above, Concerning the WBC was significantlydecreased in the totals and increased in monocytes count for the study when giving methotrexate while reversed when giving omega7 with this drug.

Conclusion

Omega7 has a significant protective role in Methotrexate-induce Blood Toxicity in Mice.

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Keywords

Methotrexate, Omega-7, RBCs, WBCs.

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