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Research Journal of Pharmacy and Technology
Year : 2023, Volume : 16, Issue : 4
First page : ( 1685) Last page : ( 1688)
Print ISSN : 0974-3618. Online ISSN : 0974-360X.
Article DOI : 10.52711/0974-360X.2023.00276

Study of gene expression in glioblastoma hypoxic model

Sharma Deeksha1, Abha Vashistha2,*, Sharma Manish3

1Department of Biotechnology and Microbiology, Meerut Institute of Engineering and Technology, NH-58, Baghpat Road, Bypass Crossing-250005, Meerut, U.P. (India)

2University Institute of Biotechnology, Chandigarh University, NH-95, Chandigarh-Ludhiana Highway-140413, Mohali, Punjab (India)

3Pioneer Center of Biosciences for Advanced Training and Research, Mohan Nagar - 201007, Ghaziabad, U.P. (India)

*Corresponding Author E-mail: abhavashistha29@gmail.com

Online Published on 13 October, 2023.

Abstract

Inspite of multi model cure therapy the mortality rate among the patients of brain tumor always remain high.The low survival rate is due to the ability of these tumors to recur quickly and aggressively. The cellular, genetic, and epigenetic heterogeneity of Glioblastoma multiforme tumors makes designing targeted treatments difficult. Within the tumor there are distinct subpopulations of cells that are stem-like in their behavior and are critical as therapeutic target. The regulation of these cells at molecular level is not yet properly studied. The presence of hypoxic environment controls the stem cells and slower down the progression of tumor. The goal of this work is to use PCR to determine gene expression in glial cells that have been exposed to hypoxia.

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Keywords

Glioblastoma, Hypoxia, HIF, Gene expression, GLUT-1, PBS, Therapeutic resistance.

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