Qualitative phytochemical screening and effectiveness analysis of Batissa violacea celebensis martens 1897 crude extract against antioxidant and cytotoxic activity Sri Rasyid Anggarini1,2,*, Bintang Maria3, As'ad Suryani4, Miskad Upik5, Minhajat Rahmawati6, Surya Ridwan Adi7 1Doctoral Program, Graduate School of Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia 2D-IV Medical Laboratory Technology Study ProgramMandala Waluya Health Science College 3Department of Biochemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, Indonesia 4Department of Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia 5Department of Pathology Anatomy, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia 6Department of Internal Medicine, Division of Hematology Medical Oncology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia 7Department of Environmental Science, Faculty of Forestry and Environmental ScienceHalu Oleo UniversityKendariSoutheast Sulawesi, Indonesia *Corresponding Author E-mail: anggarini.09@gmail.com
Online Published on 13 June, 2022. Abstract The aim of this study was to conduct phytochemical tests aimed at identifying the bioactive components present in the powder crude extract of pokea shell can be applied in analyzing antioxidant activity and testing cytotoxic activity. This study consists of phytochemical tests, including alkaloids, steroids, triterpenoids, saponins, and flavonoids. The antioxidant activity measured by the DPPH method from the crude extract of the pokea shell is still relatively weak but the crude extract of methanol of the boiled and dried pokea shells in toxic category so potentially as a cytotoxic compound. The result of phytochemical test of crude extract of pokea shell meat, is extract of fresh and boiled pokea shell contain flavonoid, saponin, steroid and triterpenoid while extract of dried pokea shell only contain saponin, steroid and triterpenoid. The result of the antioxidant activity test of crude extract of pokea shell shows that the crude extract of dried pokea shell has the highest value of antioxidant activity. The result of the toxicity test of crude extract pokea shell boiled and dried pokea shell have an LC50 value of less than 1000 ppm. These values indicate that the extract of the boiled and dried Pokea shell is showed potential for development as an alternative anticancer agents in the future. Top Keywords Phytochemical, Antioxidant, Cytotoxicity, Pokea shell, Crude extract. Top |