Depiction of chitinase extracted from Serratia marcescens su05 on agriculturally important fungi Narendrakumar G.*, Namasivayam Karthick Raja S, Saravanan Santhosh M. Department of Biotechnology, School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Chennai600119, Tamil Nadu, India *Corresponding Author E-mail: gnaren22@gmail.com
Online published on 25 August, 2021. Abstract Traditional method of controlling fungi and use of chemical pesticides, which have already been proved harmful to the environment. Hence is a potential alternative in biological control using chitinase enzyme. Thus, the necessity to achieve large scale, cost effective production of active preparations of this biopesticides has been increased. Serratia marcescens is a Gram-negative bacilli isolated from soil and identified using basic biochemical test and 16s rRNA sequencing and accession number KX002030 was obtained, that produces chitinase. This enzyme will be optimized for maximum production at various factors and media components. The extracted and partially purified enzyme (ammonium sulphate and dialysis) will be used in the analysis of for the effect of cell wall destruction of fungi pathogens. Biocontrol potential of Chitinase, dual plate assay, hyphal fragmentation test were analyzed against Alternaria alternata. Top Keywords Serratia marcescens SU05, Chitinase, Alternaria alternata. Top |