In-vitro screening for cytotoxic activity of herbal plant extracts on N2a cell lines Neelamma G1*, Rasheed Shaik Harun2, Vinukonda Anjaneyulu3, Aruna M2 1Department of Pharmacognosy, Srikrupa Institute of Pharmaceutical Sciences, Velikatta, Kondapak, Siddipet, Telangana, India 2Department of Pharmaceutics, Srikrupa Institute of Pharmaceutical Sciences, Velikatta, Kondapak, Siddipet, Telangana, India 3Team Lead, MS & T, Technical Operations, Alembic Pharmaceuticals, Vadodara, India. *Corresponding Author E-mail: nanisony2012@gmail.com
Online published on 3 June, 2021. Abstract Clitoria ternatea, Cucurbita maxima, Artemisia vulgaris were the Indian native plants used in the treatment of different diseases. The methanolic extract of Clitoria ternatea, petroleum ether extract of Cucurbita maxima and methanolic extract of Artemisia vulgaris were investigated using MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) assay. These extracts were assessed using N2a cell line (Neuroblastoma). Cisplatin was used as a positive control based on IC50 values. Artemisia vulgaris methanol extract have highest cytotoxicity (80.81ug/mL) and Cucurbita maxima seed extract have lowest cytotoxicity (57.655ug/mL). The comparison of IC50 of the extracts had shown the following toxicity: Cucurbita maxima petroleum ether extract <Clitoria ternatea methanolic extract <Artemisia vulgaris methanolic extract. The Cucurbita maxima showed least cytotoxicity against the tested cell lines. The results indicate that Cucurbita maxima seed extract have significant potential to be used as a natural anticancer agent in cultured N2a cell lines. Top Keywords MTT Assay, Cytotoxic activity, Cucurbita maxima, Artemisia vulgaris, Clitoria ternatea.. Top |